› 6eyo

X-ray diffraction
3.7Å resolution

Structure of extended IgE-Fc in complex with two anti-IgE Fabs

Released:
DOI: 10.2210/pdb6eyo/pdb
PDB entry 6eyo coloured by chain, front view.
PDB entry 6eyo coloured by chain, side view.
PDB entry 6eyo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for selective inhibition of immunoglobulin E-receptor interactions by an anti-IgE antibody.
Chen JB, Ramadani F, Pang MOY, Beavil RL, Holdom MD, Mitropoulou AN, Beavil AJ, Gould HJ, Chang TW, Sutton BJ, McDonnell JM, Davies AM
Sci Rep 8 11548 (2018)
PMID: 30069035

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208613 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Immunoglobulin heavy constant epsilon Chains: A, B
Molecule details ›
Chains: A, B
Length: 327 amino acids
Theoretical weight: 36.35 KDa
Source organism: Homo sapiens
Expression system: Mus musculus
UniProt:
  • Canonical: P01854 (Residues: 104-427; Coverage: 59%)
Gene name: IGHE
Sequence domains: Immunoglobulin C1-set domain
8D6 Fab heavy chain Chains: H, I
Molecule details ›
Chains: H, I
Length: 227 amino acids
Theoretical weight: 24.56 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
8D6 Fab light chain Chains: L, M
Molecule details ›
Chains: L, M
Length: 218 amino acids
Theoretical weight: 23.8 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 119.483Å b: 119.601Å c: 132.994Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.247 0.288
Expression systems:
  • Mus musculus
  • Homo sapiens

Quick links

Citations

5 review citations

IgE Antibodies: From Structure to Function and Clinical Translation.
Sutton et al. (2019)
4 more

3 mentions without citation

Immunoglobulins or Antibodies: IMGT® Bridging Genes, Structures and Functions.
Lefranc et al. (2020)
2 more