› 6ezo

Electron Microscopy
4.1Å resolution

Eukaryotic initiation factor EIF2B in complex with ISRIB

Released:
DOI: 10.2210/pdb6ezo/pdb
PDB entry 6ezo coloured by chain, front view.
PDB entry 6ezo coloured by chain, side view.
PDB entry 6ezo coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Binding of ISRIB reveals a regulatory site in the nucleotide exchange factor eIF2B.
Zyryanova AF, Weis F, Faille A, Alard AA, Crespillo-Casado A, Sekine Y, Harding HP, Allen F, Parts L, Fromont C, Fischer PM, Warren AJ, Ron D
Science 359 1533-1536 (2018)
PMID: 29599245
Related structures: EMD-4162

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156021 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Translation initiation factor eIF2B subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 305 amino acids
Theoretical weight: 33.75 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q14232 (Residues: 1-305; Coverage: 100%)
Gene names: EIF2B1, EIF2BA
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF2B subunit beta Chains: C, D
Molecule details ›
Chains: C, D
Length: 373 amino acids
Theoretical weight: 41.76 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P49770 (Residues: 5-351; Coverage: 99%)
Gene names: EIF2B2, EIF2BB
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF2B subunit gamma Chains: E, F
Molecule details ›
Chains: E, F
Length: 452 amino acids
Theoretical weight: 50.3 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9NR50 (Residues: 1-452; Coverage: 100%)
Gene name: EIF2B3
Sequence domains: Nucleotidyl transferase
Translation initiation factor eIF2B subunit delta Chains: G, H
Molecule details ›
Chains: G, H
Length: 523 amino acids
Theoretical weight: 57.64 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q9UI10 (Residues: 1-523; Coverage: 100%)
Gene names: EIF2B4, EIF2BD
Sequence domains: Initiation factor 2 subunit family
Translation initiation factor eIF2B subunit epsilon Chains: I, J
Molecule details ›
Chains: I, J
Length: 721 amino acids
Theoretical weight: 80.47 KDa
Source organism: Homo sapiens
UniProt:
  • Canonical: Q13144 (Residues: 1-721; Coverage: 100%)
Gene names: EIF2B5, EIF2BE
Sequence domains: eIF4-gamma/eIF5/eIF2-epsilon

Ligands and Environments

1 bound ligand:
Ligand C7B 1 x C7B
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-4162
Expression system: Homo sapiens

Quick links

Citations

30 review citations

The integrated stress response: From mechanism to disease.
Costa-Mattioli et al. (2020)
29 more

4 mentions without citation

Infinite Assembly of Folded Proteins in Evolution, Disease, and Engineering.
Garcia-Seisdedos et al. (2019)
3 more