› 6f0t

X-ray diffraction
1.74Å resolution

Crystal structure of Pizza6-SFW

Released:
DOI: 10.2210/pdb6f0t/pdb
PDB entry 6f0t coloured by chain, front view.
PDB entry 6f0t coloured by chain, side view.
PDB entry 6f0t coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Design of tryptophan-containing mutants of the symmetrical Pizza protein for biophysical studies.
Noguchi H, Mylemans B, De Zitter E, Van Meervelt L, Tame JRH, Voet A
Biochem Biophys Res Commun 497 1038-1042 (2018)
PMID: 29481797

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pizza6-SFW Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 256 amino acids
Theoretical weight: 25.84 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: TolB, C-terminal domain

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P1
Unit cell:
a: 32.529Å b: 69.454Å c: 78.71Å
α: 65.36° β: 89.8° γ: 77.16°
R-values:
R R work R free
0.194 0.192 0.233
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Influence of circular permutations on the structure and stability of a six-fold circular symmetric designer protein.
Mylemans et al. (2020)
2 more