› 6f2r

X-ray diffraction
3.9Å resolution

A heterotetramer of human HspB2 and HspB3

Released:
DOI: 10.2210/pdb6f2r/pdb
PDB entry 6f2r coloured by chain, front view.
PDB entry 6f2r coloured by chain, side view.
PDB entry 6f2r coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Terminal Regions Confer Plasticity to the Tetrameric Assembly of Human HspB2 and HspB3.
Clark AR, Vree Egberts W, Kondrat FDL, Hilton GR, Ray NJ, Cole AR, Carver JA, Benesch JLP, Keep NH, Boelens WC, Slingsby C
J Mol Biol 430 3297-3310 (2018)
PMID: 29969581

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
PDBe Complex ID:
PDB-CPX-219537 (preferred)
Entry contents:
9 distinct polypeptide molecules
Macromolecules (9 distinct):
Heat shock protein beta-2 Chains: A, E, I
Molecule details ›
Chains: A, E, I
Length: 213 amino acids
Theoretical weight: 22.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16082 (Residues: 1-182; Coverage: 100%)
Gene name: HSPB2
Sequence domains:
Heat shock protein beta-2 Chains: C, G, K
Molecule details ›
Chains: C, G, K
Length: 203 amino acids
Theoretical weight: 22.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q16082 (Residues: 1-182; Coverage: 100%)
Gene name: HSPB2
Sequence domains:
Heat shock protein beta-2 Chains: D, F, J
Molecule details ›
Chains: D, F, J
Length: 182 amino acids
Theoretical weight: 20.26 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q16082 (Residues: 1-182; Coverage: 100%)
Gene name: HSPB2
Sequence domains:
Structure domains: Immunoglobulin-like
Heat shock protein beta-3 Chains: Q, T, V
Molecule details ›
Chains: Q, T, V
Length: 161 amino acids
Theoretical weight: 18.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12988 (Residues: 1-149; Coverage: 99%)
Gene names: HSP27, HSPB3, HSPL27
Sequence domains: Hsp20/alpha crystallin family
Unknown peptide from HspB2 or HspB3 Chain: M
Molecule details ›
Chain: M
Length: 10 amino acids
Theoretical weight: 869 Da
Source organism: Homo sapiens
Expression system: Escherichia coli
Unknown peptide from HspB2 or HspB3 Chain: N
Molecule details ›
Chain: N
Length: 7 amino acids
Theoretical weight: 614 Da
Source organism: Homo sapiens
Expression system: Escherichia coli
Unknown peptide from HspB2 or HspB3 Chain: O
Molecule details ›
Chain: O
Length: 6 amino acids
Theoretical weight: 529 Da
Source organism: Homo sapiens
Expression system: Escherichia coli
Unknown peptide from HspB2 or HspB3 Chains: 1, 2, 3
Molecule details ›
Chains: 1, 2, 3
Length: 5 amino acids
Theoretical weight: 444 Da
Source organism: Homo sapiens
Expression system: Escherichia coli
Unknown peptide from HspB2 or HspB3 Chains: W, X, Y
Molecule details ›
Chains: W, X, Y
Length: 12 amino acids
Theoretical weight: 1.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P31
Unit cell:
a: 177.139Å b: 177.139Å c: 126.461Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.298 0.297 0.313
Expression systems:
  • Escherichia coli
  • Homo sapiens

Quick links

Citations

6 review citations

Small heat shock proteins: Simplicity meets complexity.
Haslbeck et al. (2019)
5 more

8 mentions without citation

Mechanisms of Small Heat Shock Proteins.
Janowska et al. (2019)
7 more