› 6f3k

Hybrid

Combined solid-state NMR, solution-state NMR and EM data for structure determination of the tetrahedral aminopeptidase TET2 from P. horikoshii

Released:
DOI: 10.2210/pdb6f3k/pdb
PDB entry 6f3k coloured by chain, ensemble of 10 models, front view.
PDB entry 6f3k coloured by chain, ensemble of 10 models, side view.
PDB entry 6f3k coloured by chain, ensemble of 10 models, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Integrated NMR and cryo-EM atomic-resolution structure determination of a half-megadalton enzyme complex.
Gauto DF, Estrozi LF, Schwieters CD, Effantin G, Macek P, Sounier R, Sivertsen AC, Schmidt E, Kerfah R, Mas G, Colletier JP, Güntert P, Favier A, Schoehn G, Schanda P, Boisbouvier J
Nat Commun 10 2697 (2019)
PMID: 31217444
Related structures: EMD-4179

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129829 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetrahedral aminopeptidase Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 39.07 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O59196 (Residues: 1-353; Coverage: 100%)
Gene names: PH1527, frvX
Sequence domains: M42 glutamyl aminopeptidase

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Experimental Method: Solid-state NMR, Solution NMR, Electron Microscopy
Resolution: 4.1Å
Relevant EMDB volumes: EMD-4179
Expression system: Escherichia coli

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Citations

8 review citations

FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices.
Lerner et al. (2021)
7 more

4 mentions without citation

1H-Detected Biomolecular NMR under Fast Magic-Angle Spinning.
Le Marchand et al. (2022)
3 more