› 6f3q

X-ray diffraction
1.45Å resolution

Crystal structure of S-adenosyl-L-homocysteine hydrolase from Pseudomonas aeruginosa in complex with adenine and Rb+ cation

Released:
DOI: 10.2210/pdb6f3q/pdb
PDB entry 6f3q coloured by chain, front view.
PDB entry 6f3q coloured by chain, side view.
PDB entry 6f3q coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
Metal-cation regulation of enzyme dynamics is a key factor influencing the activity of S-adenosyl-L-homocysteine hydrolase from Pseudomonas aeruginosa.
Czyrko J, Sliwiak J, Imiolczyk B, Gdaniec Z, Jaskolski M, Brzezinski K
OpenAccess logo Sci Rep 8 11334 (2018)
PMID: 30054521

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191446 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosylhomocysteinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 472 amino acids
Theoretical weight: 51.74 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9I685 (Residues: 1-469; Coverage: 100%)
Gene names: PA0432, ahcY, sahH
Sequence domains:

Ligands and Environments

6 bound ligands:
Ligand RB 5 x RB
Ligand PO4 8 x PO4
Ligand NAD 4 x NAD
Ligand ADE 4 x ADE
Ligand PEG 1 x PEG
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2
Unit cell:
a: 170.492Å b: 99.442Å c: 111.714Å
α: 90° β: 101.93° γ: 90°
R-values:
R R work R free
0.093 0.093 0.124
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Functional and Pathological Roles of AHCY.
Vizán et al. (2021)
1 more

2 mentions without citation

S-adenosyl-l-homocysteine Hydrolase: A Structural Perspective on the Enzyme with Two Rossmann-Fold Domains.
Brzezinski K. (2020)
1 more