› 6f50

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF KETOSTEROID ISOMERASE DOUBLE VARIANT V88I/L99V

Released:
DOI: 10.2210/pdb6f50/pdb
PDB entry 6f50 coloured by chain, front view.
PDB entry 6f50 coloured by chain, side view.
PDB entry 6f50 coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Engineering the "Missing Link" in Biosynthetic (-)-Menthol Production: Bacterial Isopulegone Isomerase.
Currin A, Dunstan MS, Johannissen LO, Hollywood KA, Vinaixa M, Jervis AJ, Swainston N, Rattray NJW, Gardiner JM, Kell DB, Takano E, Toogood HS, Scrutton NS
ACS Catal 8 2012-2020 (2018)
PMID: 29750129

Function and Biology Details

Reaction catalysed: 
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139593 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Steroid Delta-isomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 125 amino acids
Theoretical weight: 13.79 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: P07445 (Residues: 3-127; Coverage: 95%)
Gene name: ksi
Sequence domains: SnoaL-like domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 35.692Å b: 73.539Å c: 95.414Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.227
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Strategies for the production of biochemicals in bioenergy crops.
Lin et al. (2020)
2 more

1 mention without citation

Engineering the "Missing Link" in Biosynthetic (-)-Menthol Production: Bacterial Isopulegone Isomerase.
Currin et al. (2018)