› 6f59

X-ray diffraction
2.15Å resolution

Crystal structure of human Brachyury (T) G177D variant in complex with DNA

Released:
DOI: 10.2210/pdb6f59/pdb
PDB entry 6f59 coloured by chain, front view.
PDB entry 6f59 coloured by chain, side view.
PDB entry 6f59 coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Newman JA, Gavard AE, Krojer T, Shrestha L, Burgess-Brown NA, von Delft F, Arrowsmith CH, Edwards A, Bountra C, Gileadi O

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119879 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
T-box transcription factor T Chains: A, B
Molecule details ›
Chains: A, B
Length: 192 amino acids
Theoretical weight: 22.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15178 (Residues: 41-224; Coverage: 42%)
Gene names: T, TBXT
Sequence domains: T-box
Structure domains: Transcription factor, T-box
DNA (26-MER) Chains: C, D
Molecule details ›
Chains: C, D
Length: 26 nucleotides
Theoretical weight: 7.99 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand NA 1 x NA
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P4322
Unit cell:
a: 75.27Å b: 75.27Å c: 288.468Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.221 0.252
Expression systems:
  • Escherichia coli
  • Not provided

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