› 6f9o

X-ray diffraction
1.05Å resolution

Crystal structure of cold-adapted haloalkane dehalogenase DpcA from Psychrobacter cryohalolentis K5

Released:
DOI: 10.2210/pdb6f9o/pdb
PDB entry 6f9o coloured by chain, front view.
PDB entry 6f9o coloured by chain, side view.
PDB entry 6f9o coloured by chain, top view.
Source organism: Psychrobacter cryohalolentis K5
Primary publication:
Crystal structure of the cold-adapted haloalkane dehalogenase DpcA from Psychrobacter cryohalolentis K5.
Tratsiak K, Prudnikova T, Drienovska I, Damborsky J, Brynda J, Pachl P, Kuty M, Chaloupkova R, Rezacova P, Kuta Smatanova I
Acta Crystallogr F Struct Biol Commun 75 324-331 (2019)
PMID: 31045561

Function and Biology Details

Reaction catalysed: 
1-haloalkane + H(2)O = a primary alcohol + halide
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173003 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haloalkane dehalogenase Chain: A
Molecule details ›
Chain: A
Length: 309 amino acids
Theoretical weight: 34.91 KDa
Source organism: Psychrobacter cryohalolentis K5
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q1QBB9 (Residues: 1-303; Coverage: 100%)
Gene names: Pcryo_1253, dhmA
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

3 bound ligands:
Ligand CL 2 x CL
Ligand PEG 5 x PEG
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P21
Unit cell:
a: 41.332Å b: 79.451Å c: 43.489Å
α: 90° β: 94.98° γ: 90°
R-values:
R R work R free
0.111 0.11 0.132
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

3 citation in other articles

Response of Lactiplantibacillus plantarum NMGL2 to Combinational Cold and Acid Stresses during Storage of Fermented Milk as Analyzed by Data-Independent Acquisition Proteomics.
Zhang et al. (2021)
2 more

1 mention without citation

Crystal structure of the cold-adapted haloalkane dehalogenase DpcA from Psychrobacter cryohalolentis K5.
Tratsiak et al. (2019)