› 6fat

X-ray diffraction
2.3Å resolution

The crystal structure of a feruloyl esterase C from Fusarium oxysporum.

Released:
DOI: 10.2210/pdb6fat/pdb
PDB entry 6fat coloured by chain, front view.
PDB entry 6fat coloured by chain, side view.
PDB entry 6fat coloured by chain, top view.
Source organism: Fusarium oxysporum
Primary publication:
The crystal structure of a Fusarium oxysporum feruloyl esterase that belongs to the tannase family
Dimarogona M, Topakas E, Christakopoulos P, Chrysina ED
FEBS Lett. (2020)

Function and Biology Details

Reaction catalysed: 
Feruloyl-polysaccharide + H(2)O = ferulate + polysaccharide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103351 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (6 distinct):
Carboxylic ester hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 528 amino acids
Theoretical weight: 58.52 KDa
Source organism: Fusarium oxysporum
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A0A1D3S5H0 (Residues: 36-563; Coverage: 94%)
Gene name: faeC
Sequence domains: Tannase and feruloyl esterase

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
2 bound ligands:
Ligand NAG 2 x NAG
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: P21
Unit cell:
a: 67.536Å b: 87.467Å c: 106.565Å
α: 90° β: 106.18° γ: 90°
R-values:
R R work R free
0.208 0.207 0.235
Expression system: Komagataella pastoris

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