› 6fb1

X-ray diffraction
3.02Å resolution

Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with its target DNA (Haemoglobin beta subunit gene) in the presence of Magnesium

Released:
DOI: 10.2210/pdb6fb1/pdb
PDB entry 6fb1 coloured by chain, front view.
PDB entry 6fb1 coloured by chain, side view.
PDB entry 6fb1 coloured by chain, top view.
Source organism: Chlamydomonas reinhardtii
Primary publication:
Understanding the indirect DNA read-out specificity of I-CreI Meganuclease.
Prieto J, Redondo P, López-Méndez B, D'Abramo M, Merino N, Blanco FJ, Duchateau P, Montoya G, Molina R
Sci Rep 8 10286 (2018)
PMID: 29980759

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119884 (preferred)
Entry contents:
2 distinct polypeptide molecules
4 distinct DNA molecules
Macromolecules (6 distinct):
DNA endonuclease I-CreI Chain: A
Molecule details ›
Chain: A
Length: 154 amino acids
Theoretical weight: 17.64 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
UniProt:
  • Canonical: P05725 (Residues: 2-155; Coverage: 95%)
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
DNA endonuclease I-CreI Chain: B
Molecule details ›
Chain: B
Length: 154 amino acids
Theoretical weight: 17.58 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
UniProt:
  • Canonical: P05725 (Residues: 2-155; Coverage: 95%)
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
DNA (5'-D(*TP*CP*AP*GP*AP*CP*TP*TP*CP*TP*CP*CP*AP*C)-3') Chain: D
Molecule details ›
Chain: D
Length: 14 nucleotides
Theoretical weight: 4.18 KDa
DNA (5'-D(*TP*CP*TP*GP*AP*CP*TP*CP*CP*TP*GP*TP*GP*G)-3') Chain: F
Molecule details ›
Chain: F
Length: 14 nucleotides
Theoretical weight: 4.26 KDa
DNA (5'-D(P*AP*GP*GP*AP*GP*TP*CP*AP*GP*A)-3') Chain: E
Molecule details ›
Chain: E
Length: 10 nucleotides
Theoretical weight: 3.12 KDa
DNA (5'-D(P*AP*GP*AP*AP*GP*TP*CP*TP*GP*A)-3') Chain: G
Molecule details ›
Chain: G
Length: 10 nucleotides
Theoretical weight: 3.09 KDa

Ligands and Environments

1 bound ligand:
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 45.09Å b: 66.6Å c: 91.78Å
α: 90° β: 96.69° γ: 90°
R-values:
R R work R free
0.21 0.208 0.252
Expression system: Escherichia coli

Quick links

Citations

7 review citations

The genome editing revolution: review.
Khalil AM. (2020)
6 more