› 6fb7

X-ray diffraction
2.69Å resolution

Crystal Structure of the I-CreI Homing Endonuclease D75N variant in complex with its target DNA in the presence of Manganese

Released:
DOI: 10.2210/pdb6fb7/pdb
PDB entry 6fb7 coloured by chain, front view.
PDB entry 6fb7 coloured by chain, side view.
PDB entry 6fb7 coloured by chain, top view.
Source organisms:
Primary publication:
Understanding the indirect DNA read-out specificity of I-CreI Meganuclease.
Prieto J, Redondo P, López-Méndez B, D'Abramo M, Merino N, Blanco FJ, Duchateau P, Montoya G, Molina R
Sci Rep 8 10286 (2018)
PMID: 29980759

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119888 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA endonuclease I-CreI Chains: A, B
Molecule details ›
Chains: A, B
Length: 152 amino acids
Theoretical weight: 17.52 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: synthetic construct
UniProt:
  • Canonical: P05725 (Residues: 2-153; Coverage: 93%)
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
DNA (5'-D(*TP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*AP*C)-3') Chains: D, F
Molecule details ›
Chains: D, F
Length: 14 nucleotides
Theoretical weight: 4.25 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*GP*AP*CP*GP*TP*TP*TP*TP*GP*A)-3') Chains: E, G
Molecule details ›
Chains: E, G
Length: 10 nucleotides
Theoretical weight: 3.08 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MN 3 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21212
Unit cell:
a: 71.504Å b: 169.153Å c: 49.015Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.163 0.211
Expression systems:
  • synthetic construct
  • Not provided

Quick links

Citations

7 review citations

The genome editing revolution: review.
Khalil AM. (2020)
6 more