› 6fb8

X-ray diffraction
2.45Å resolution

Crystal Structure of the I-CreI Homing Endonuclease D75N variant in complex with an altered version of its target DNA at 5NNN region in the presence of Magnesium

Released:
DOI: 10.2210/pdb6fb8/pdb
PDB entry 6fb8 coloured by chain, front view.
PDB entry 6fb8 coloured by chain, side view.
PDB entry 6fb8 coloured by chain, top view.
Source organisms:
Primary publication:
Understanding the indirect DNA read-out specificity of I-CreI Meganuclease.
Prieto J, Redondo P, López-Méndez B, D'Abramo M, Merino N, Blanco FJ, Duchateau P, Montoya G, Molina R
Sci Rep 8 10286 (2018)
PMID: 29980759

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119889 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA endonuclease I-CreI Chains: A, B
Molecule details ›
Chains: A, B
Length: 154 amino acids
Theoretical weight: 17.66 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Escherichia coli
UniProt:
  • Canonical: P05725 (Residues: 2-153; Coverage: 93%)
Sequence domains: LAGLIDADG endonuclease
Structure domains: Homing endonucleases
DNA (5'-D(*TP*CP*AP*AP*AP*AP*CP*GP*TP*CP*GP*TP*AP*CP*GP*CP*AP*GP*TP*TP*TP*TP*GP*A)-3') Chain: D
Molecule details ›
Chain: D
Length: 24 nucleotides
Theoretical weight: 7.37 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(*TP*CP*AP*AP*AP*AP*CP*TP*GP*CP*GP*TP*AP*CP*GP*AP*CP*GP*TP*TP*TP*TP*GP*A)-3') Chain: C
Molecule details ›
Chain: C
Length: 24 nucleotides
Theoretical weight: 7.37 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand PGO 4 x PGO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P43
Unit cell:
a: 79.48Å b: 79.48Å c: 125.76Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.186 0.224
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

The genome editing revolution: review.
Khalil AM. (2020)
6 more