› 6fbk

X-ray diffraction
1.74Å resolution

Crystal structure of the human WNK2 CCT-like 1 domain in complex with a WNK1 RFXV peptide

Released:
DOI: 10.2210/pdb6fbk/pdb
PDB entry 6fbk coloured by chain, front view.
PDB entry 6fbk coloured by chain, side view.
PDB entry 6fbk coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Pinkas DM, Bufton JC, Burgess-Brown NA, Arrowsmith CH, Edwards AM, Bountra C, Bullock AN

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190605 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase WNK2 Chain: A
Molecule details ›
Chain: A
Length: 98 amino acids
Theoretical weight: 11.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y3S1 (Residues: 454-549; Coverage: 4%)
Gene names: KIAA1760, P/OKcl.13, PRKWNK2, SDCCAG43, WNK2
Sequence domains: Oxidative-stress-responsive kinase 1 C-terminal domain
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Serine/threonine-protein kinase WNK1 Chain: P
Molecule details ›
Chain: P
Length: 23 amino acids
Theoretical weight: 2.61 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9H4A3 (Residues: 1247-1269; Coverage: 1%)
Gene names: HSN2, KDP, KIAA0344, PRKWNK1, WNK1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: I41
Unit cell:
a: 78.047Å b: 78.047Å c: 37.736Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.287 0.284 0.334
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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