› 6fd3

X-ray diffraction
1.52Å resolution

Thiophosphorylated PAK3 kinase domain

Released:
DOI: 10.2210/pdb6fd3/pdb
PDB entry 6fd3 coloured by chain, front view.
PDB entry 6fd3 coloured by chain, side view.
PDB entry 6fd3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Solution structures and biophysical analysis of full-length group A PAKs reveal they are monomeric and auto-inhibited in cis.
Sorrell FJ, Kilian LM, Elkins JM
Biochem J 476 1037-1051 (2019)
PMID: 30858169

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131172 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase PAK 3 Chain: A
Molecule details ›
Chain: A
Length: 300 amino acids
Theoretical weight: 33.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75914 (Residues: 261-559; Coverage: 54%)
Gene names: OPHN3, PAK3
Sequence domains: Protein kinase domain

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand ADP 1 x ADP
Ligand EDO 2 x EDO
1 modified residue:
Ligand T8L 1 x T8L

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: I41
Unit cell:
a: 108.825Å b: 108.825Å c: 57.934Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.175 0.19
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Targeting Rho GTPase Signaling Networks in Cancer.
Clayton et al. (2020)
6 more

3 mentions without citation

Novel PAK3 gene missense variant associated with two Chinese siblings with intellectual disability: a case report.
Qian et al. (2020)
2 more