› 6fgc

X-ray diffraction
1.5Å resolution

Crystal structure of Gephyrin E domain in complex with Artesunate

Released:
DOI: 10.2210/pdb6fgc/pdb
PDB entry 6fgc coloured by chain, front view.
PDB entry 6fgc coloured by chain, side view.
PDB entry 6fgc coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Elucidating the Molecular Basis for Inhibitory Neurotransmission Regulation by Artemisinins.
Kasaragod VB, Hausrat TJ, Schaefer N, Kuhn M, Christensen NR, Tessmer I, Maric HM, Madsen KL, Sotriffer C, Villmann C, Kneussel M, Schindelin H
Neuron 101 673-689.e11 (2019)
PMID: 30704910

Function and Biology Details

Reactions catalysed: 
Adenylyl-molybdopterin + molybdate = molybdenum cofactor + AMP
ATP + molybdopterin = diphosphate + adenylyl-molybdopterin
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169917 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gephyrin Chain: A
Molecule details ›
Chain: A
Length: 419 amino acids
Theoretical weight: 45.65 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03555 (Residues: 350-768; Coverage: 55%)
Gene names: Gph, Gphn
Sequence domains:
Structure domains:

Ligands and Environments

8 bound ligands:
Ligand CA 1 x CA
Ligand ADP 1 x ADP
Ligand PO4 1 x PO4
Ligand MPD 1 x MPD
Ligand MRD 1 x MRD
Ligand ACT 10 x ACT
Ligand D95 1 x D95
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: I222
Unit cell:
a: 87.1Å b: 99.22Å c: 113.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 0.143 0.162
Expression system: Escherichia coli

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Citations

1 review citation

Looking for Novelty in an "Old" Receptor: Recent Advances Toward Our Understanding of GABAARs and Their Implications in Receptor Pharmacology.
Castellano et al. (2020)
8 other articles

3 mentions without citation

Structure of Heteropentameric GABAA Receptors and Receptor-Anchoring Properties of Gephyrin.
Kasaragod et al. (2019)
2 more