› 6fhg

X-ray diffraction
1.95Å resolution

Crystal structure of the Ts2631 endolysin from Thermus scotoductus phage with the unique N-terminal moiety responsible for peptidoglycan anchoring

Released:
DOI: 10.2210/pdb6fhg/pdb
PDB entry 6fhg coloured by chain, front view.
PDB entry 6fhg coloured by chain, side view.
PDB entry 6fhg coloured by chain, top view.
Source organism: Thermus phage 2631
Primary publication:
Structure and function of the Ts2631 endolysin of Thermus scotoductus phage vB_Tsc2631 with unique N-terminal extension used for peptidoglycan binding.
Plotka M, Sancho-Vaello E, Dorawa S, Kaczorowska AK, Kozlowski LP, Kaczorowski T, Zeth K
Sci Rep 9 1261 (2019)
PMID: 30718611

Function and Biology Details

Reaction catalysed: 
Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100688 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetylmuramoyl-L-alanine amidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 156 amino acids
Theoretical weight: 18.1 KDa
Source organism: Thermus phage 2631
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A088FLK9 (Residues: 1-156; Coverage: 100%)
Sequence domains: N-acetylmuramoyl-L-alanine amidase

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P212121
Unit cell:
a: 53.579Å b: 56.094Å c: 116.715Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.203 0.247
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The Age of Phage: Friend or Foe in the New Dawn of Therapeutic and Biocontrol Applications?
Hassan et al. (2021)
2 more

3 mentions without citation

Structure and function of the Ts2631 endolysin of Thermus scotoductus phage vB_Tsc2631 with unique N-terminal extension used for peptidoglycan binding.
Plotka et al. (2019)
2 more