› 6fho

X-ray diffraction
1.75Å resolution

Crystal structure of pqsL, a probable FAD-dependent monooxygenase from Pseudomonas aeruginosa - new refinement

Released:
DOI: 10.2210/pdb6fho/pdb
PDB entry 6fho coloured by chain, front view.
PDB entry 6fho coloured by chain, side view.
PDB entry 6fho coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Primary publication:
PqsL uses reduced flavin to produce 2-hydroxylaminobenzoylacetate, a preferred PqsBC substrate in alkyl quinolone biosynthesis in Pseudomonas aeruginosa.
Drees SL, Ernst S, Belviso BD, Jagmann N, Hennecke U, Fetzner S
J Biol Chem 293 9345-9357 (2018)
PMID: 29669807

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191062 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD-binding domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 399 amino acids
Theoretical weight: 44.09 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HWJ1 (Residues: 1-398; Coverage: 100%)
Gene names: PA4190, pqsL
Sequence domains: FAD binding domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FAD 1 x FAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 47.077Å b: 63.257Å c: 128.628Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.219
Expression system: Escherichia coli

Quick links

Citations

4 review citations

The Pseudomonas Quinolone Signal (PQS): Not Just for Quorum Sensing Anymore.
Lin et al. (2018)
3 more

2 mentions without citation

Enzyme evolution in fungal indole alkaloid biosynthesis.
Fraley et al. (2020)
1 more