› 6fhs

Electron Microscopy
3.75Å resolution

CryoEM Structure of INO80core

Released:
DOI: 10.2210/pdb6fhs/pdb
PDB entry 6fhs coloured by chain, front view.
PDB entry 6fhs coloured by chain, side view.
PDB entry 6fhs coloured by chain, top view.
Source organism: Thermochaetoides thermophila DSM 1495
Primary publication:
Structural basis for ATP-dependent chromatin remodelling by the INO80 complex.
Eustermann S, Schall K, Kostrewa D, Lakomek K, Strauss M, Moldt M, Hopfner KP
Nature 556 386-390 (2018)
PMID: 29643509
Related structures: EMD-4264

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-123882 (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
RuvB-like helicase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 462 amino acids
Theoretical weight: 50.45 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Trichoplusia ni
UniProt:
  • Canonical: G0RYI5 (Residues: 1-462; Coverage: 100%)
Gene name: CTHT_0006820
Sequence domains:
RuvB-like helicase Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 488 amino acids
Theoretical weight: 53.21 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Trichoplusia ni
UniProt:
  • Canonical: G0RYC2 (Residues: 1-488; Coverage: 100%)
Gene name: CTHT_0006170
Sequence domains:
Ino80 Chain: G
Molecule details ›
Chain: G
Length: 1107 amino acids
Theoretical weight: 127.53 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Trichoplusia ni
INO80 complex subunit B-like conserved region domain-containing protein Chain: H
Molecule details ›
Chain: H
Length: 491 amino acids
Theoretical weight: 53.26 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Trichoplusia ni
UniProt:
  • Canonical: G0RY01 (Residues: 1-491; Coverage: 100%)
Gene name: CTHT_0004910
Sequence domains: PAPA-1-like conserved region
Vps72/YL1 C-terminal domain-containing protein Chain: I
Molecule details ›
Chain: I
Length: 219 amino acids
Theoretical weight: 23.13 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Trichoplusia ni
UniProt:
  • Canonical: G0S590 (Residues: 1-219; Coverage: 100%)
Gene name: CTHT_0032670
Sequence domains: YL1 nuclear protein C-terminal domain
Arp5 Chain: J
Molecule details ›
Chain: J
Length: 769 amino acids
Theoretical weight: 87.77 KDa
Source organism: Thermochaetoides thermophila DSM 1495
Expression system: Trichoplusia ni
UniProt:
  • Canonical: G0S589 (Residues: 98-866; Coverage: 89%)
Gene name: CTHT_0032660
Sequence domains: Actin

Ligands and Environments

2 bound ligands:
Ligand ADP 6 x ADP
Ligand ATP 1 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.754Å
Relevant EMDB volumes: EMD-4264
Expression system: Trichoplusia ni

Quick links

Citations

26 review citations

Chromatin Remodelers in the 3D Nuclear Compartment.
Magaña-Acosta et al. (2020)
25 more

3 mentions without citation

AAA ATPases as therapeutic targets: Structure, functions, and small-molecule inhibitors.
Zhang et al. (2021)
2 more