› 6fj4

X-ray diffraction
1.7Å resolution

Structure of FAE solved by SAD from data collected at the peak of the Selenium absorption edge on ID30B

Released:
DOI: 10.2210/pdb6fj4/pdb
PDB entry 6fj4 coloured by chain, front view.
PDB entry 6fj4 coloured by chain, side view.
PDB entry 6fj4 coloured by chain, top view.
Source organism: Acetivibrio thermocellus
Primary publication:
ID30B - a versatile beamline for macromolecular crystallography experiments at the ESRF.
McCarthy AA, Barrett R, Beteva A, Caserotto H, Dobias F, Felisaz F, Giraud T, Guijarro M, Janocha R, Khadrouche A, Lentini M, Leonard GA, Lopez Marrero M, Malbet-Monaco S, McSweeney S, Nurizzo D, Papp G, Rossi C, Sinoir J, Sorez C, Surr J, Svensson O, Zander U, Cipriani F, Theveneau P, Mueller-Dieckmann C
J Synchrotron Radiat 25 1249-1260 (2018)
PMID: 29979188

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156366 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-1,4-beta-xylanase Y Chain: A
Molecule details ›
Chain: A
Length: 283 amino acids
Theoretical weight: 32.76 KDa
Source organism: Acetivibrio thermocellus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P51584 (Residues: 803-1077; Coverage: 26%)
Gene name: xynY
Sequence domains: Putative esterase
Structure domains: alpha/beta hydrolase

Ligands and Environments

3 bound ligands:
Ligand CD 5 x CD
Ligand GOL 2 x GOL
Ligand SO4 1 x SO4
2 modified residues:
Ligand MSE 8 x MSE
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P41212
Unit cell:
a: 112.023Å b: 112.023Å c: 65.865Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.161 0.16 0.178
Expression system: Escherichia coli BL21

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Citations

29 citation in other articles

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1 mention without citation

ID30B - a versatile beamline for macromolecular crystallography experiments at the ESRF.
McCarthy et al. (2018)