› 6fk0

X-ray diffraction
2.9Å resolution

Xray structure of domain-swapped cystatin E dimer

Released:
DOI: 10.2210/pdb6fk0/pdb
PDB entry 6fk0 coloured by chain, front view.
PDB entry 6fk0 coloured by chain, side view.
PDB entry 6fk0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional analysis of cystatin E reveals enzymologically relevant dimer and amyloid fibril states.
Dall E, Hollerweger JC, Dahms SO, Cui H, Häussermann K, Brandstetter H
J Biol Chem 293 13151-13165 (2018)
PMID: 29967063

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172422 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystatin-M Chains: A, B
Molecule details ›
Chains: A, B
Length: 131 amino acids
Theoretical weight: 14.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15828 (Residues: 29-149; Coverage: 100%)
Gene name: CST6
Sequence domains: Cystatin domain
Structure domains: Nuclear Transport Factor 2; Chain: A,

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P2221
Unit cell:
a: 31.62Å b: 64.07Å c: 147.73Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.268 0.268 0.273
Expression system: Escherichia coli

Quick links

Citations

4 review citations

δ-secretase in neurodegenerative diseases: mechanisms, regulators and therapeutic opportunities.
Zhang et al. (2020)
3 more

1 mention without citation

Structural and functional analysis of cystatin E reveals enzymologically relevant dimer and amyloid fibril states.
Dall et al. (2018)