› 6fko

X-ray diffraction
2.1Å resolution

Deoxyguanylosuccinate synthase (DgsS) quaternary structure with ATP, dGMP, hadacidin at 2.1 Angstrom resolution

Released:
DOI: 10.2210/pdb6fko/pdb
PDB entry 6fko coloured by chain, front view.
PDB entry 6fko coloured by chain, side view.
PDB entry 6fko coloured by chain, top view.
Source organism: Vibrio phage phiVC8
Entry authors: Sleiman D, Loc'h J, Haouz A, Kaminski PA

Function and Biology Details

Reaction catalysed: 
ATP + dGMP + L-aspartate = ADP + phosphate + 2-amino-2'-deoxy-N(6)-((2S)-succino)adenylate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124326 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N6-succino-2-amino-2'-deoxyadenylate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 363 amino acids
Theoretical weight: 40.39 KDa
Source organism: Vibrio phage phiVC8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G3FFN6 (Residues: 3-343; Coverage: 99%)
Gene names: phiVC8_p27, purZ
Sequence domains: Adenylosuccinate synthetase

Ligands and Environments

4 bound ligands:
Ligand HDA 1 x HDA
Ligand DGP 2 x DGP
Ligand FLC 1 x FLC
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P41212
Unit cell:
a: 91.97Å b: 91.97Å c: 192.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.189 0.222
Expression system: Escherichia coli BL21(DE3)

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