› 6fku

X-ray diffraction
2.4Å resolution

Structure and function of aldehyde dehydrogenase from Thermus thermophilus: An enzyme with an evolutionarily-distinct C-terminal arm (Recombinant protein with shortened C-terminal, in complex with NADP)

Released:
DOI: 10.2210/pdb6fku/pdb
Model geometry
Fit model/data
PDB entry 6fku coloured by chain, front view.
PDB entry 6fku coloured by chain, side view.
PDB entry 6fku coloured by chain, top view.
Source organism: Thermus thermophilus HB27
Primary publication:
The quaternary structure of Thermus thermophilus aldehyde dehydrogenase is stabilized by an evolutionary distinct C-terminal arm extension.
Hayes K, Noor M, Djeghader A, Armshaw P, Pembroke T, Tofail S, Soulimane T
OpenAccess logo Sci Rep 8 13327 (2018)
PMID: 30190503

Function and Biology Details

Reaction catalysed: 
An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180878 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldehyde dehydrogenase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 521 amino acids
Theoretical weight: 57.92 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q72KD3 (Residues: 2-515; Coverage: 97%)
Gene name: TT_C0513
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAP 2 x NAP
5 bound ligands:
Ligand PGE 6 x PGE
Ligand PEG 3 x PEG
Ligand PG4 1 x PG4
Ligand 1PE 3 x 1PE
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P41212
Unit cell:
a: 105.23Å b: 105.23Å c: 315.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.183 0.209
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Insights into Aldehyde Dehydrogenase Enzymes: A Structural Perspective.
Shortall et al. (2021)
9 other articles

1 mention without citation

The quaternary structure of Thermus thermophilus aldehyde dehydrogenase is stabilized by an evolutionary distinct C-terminal arm extension.
Hayes et al. (2018)