› 6fkx

X-ray diffraction
2.03Å resolution

Crystal structure of an acetyl xylan esterase from a desert metagenome

Released:
DOI: 10.2210/pdb6fkx/pdb
PDB entry 6fkx coloured by chain, front view.
PDB entry 6fkx coloured by chain, side view.
PDB entry 6fkx coloured by chain, top view.
Source organism: metagenome
Primary publication:
Structural Characterization and Directed Evolution of a Novel Acetyl Xylan Esterase Reveals Thermostability Determinants of the Carbohydrate Esterase 7 Family.
Adesioye FA, Makhalanyane TP, Vikram S, Sewell BT, Schubert WD, Cowan DA
Appl Environ Microbiol 84 (2018)
PMID: 29453256

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166716 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Acetyl xylan esterase Chain: A
Molecule details ›
Chain: A
Length: 324 amino acids
Theoretical weight: 36.04 KDa
Source organism: metagenome
Expression system: Escherichia coli BL21
Structure domains: alpha/beta hydrolase
Acetyl xylan esterase Chains: B, D, E, F
Molecule details ›
Chains: B, D, E, F
Length: 322 amino acids
Theoretical weight: 35.76 KDa
Source organism: metagenome
Expression system: Escherichia coli BL21
Structure domains: alpha/beta hydrolase
Acetyl xylan esterase Chain: C
Molecule details ›
Chain: C
Length: 319 amino acids
Theoretical weight: 35.43 KDa
Source organism: metagenome
Expression system: Escherichia coli BL21
Structure domains: alpha/beta hydrolase

Ligands and Environments

4 bound ligands:
Ligand NA 6 x NA
Ligand MES 13 x MES
Ligand SO4 1 x SO4
Ligand FMT 1 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 107.68Å b: 116.82Å c: 159.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.169 0.22
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Recent Development of Extremophilic Bacteria and Their Application in Biorefinery.
Zhu et al. (2020)
3 more

3 mentions without citation

Crystal structure and functional characterization of a cold-active acetyl xylan esterase (PbAcE) from psychrophilic soil microbe Paenibacillus sp.
Park et al. (2018)
2 more