› 6flq

Electron Microscopy
4.1Å resolution

CryoEM structure of E.coli RNA polymerase paused elongation complex bound to NusA

Released:
DOI: 10.2210/pdb6flq/pdb
PDB entry 6flq coloured by chain, front view.
PDB entry 6flq coloured by chain, side view.
PDB entry 6flq coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural Basis for NusA Stabilized Transcriptional Pausing.
Guo X, Myasnikov AG, Chen J, Crucifix C, Papai G, Takacs M, Schultz P, Weixlbaumer A
Mol Cell 69 816-827.e4 (2018)
PMID: 29499136
Related structures: EMD-4275

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
PDBe Complex ID:
PDB-CPX-119910 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (8 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 329 amino acids
Theoretical weight: 36.56 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A7Z4 (Residues: 1-329; Coverage: 100%)
Gene names: JW3257, b3295, pez, phs, rpoA, sez
Sequence domains:
DNA-directed RNA polymerase subunit beta Chain: C
Molecule details ›
Chain: C
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A8V2 (Residues: 1-1342; Coverage: 100%)
Gene names: JW3950, b3987, groN, nitB, rif, ron, rpoB, stl, stv, tabD
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chain: D
Molecule details ›
Chain: D
Length: 1407 amino acids
Theoretical weight: 155.37 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A8T7 (Residues: 1-1407; Coverage: 100%)
Gene names: JW3951, b3988, rpoC, tabB
Sequence domains:
DNA-directed RNA polymerase subunit omega Chain: E
Molecule details ›
Chain: E
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A800 (Residues: 1-91; Coverage: 100%)
Gene names: JW3624, b3649, rpoZ
Sequence domains: RNA polymerase Rpb6
Transcription termination/antitermination protein NusA Chain: F
Molecule details ›
Chain: F
Length: 495 amino acids
Theoretical weight: 54.93 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AFF6 (Residues: 1-495; Coverage: 100%)
Gene names: JW3138, b3169, nusA
Sequence domains:
DNA (31-MER) Chain: N
Molecule details ›
Chain: N
Length: 31 nucleotides
Theoretical weight: 9.68 KDa
DNA (39-MER) Chain: T
Molecule details ›
Chain: T
Length: 39 nucleotides
Theoretical weight: 11.82 KDa
RNA (5'-R(*CP*CP*UP*GP*AP*UP*CP*AP*GP*GP*CP*GP*AP*UP*GP*UP*GP*UP*GP*CP*U)-3') Chain: R
Molecule details ›
Chain: R
Length: 21 nucleotides
Theoretical weight: 6.72 KDa

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.1Å
Relevant EMDB volumes: EMD-4275
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

Organization and regulation of gene transcription.
Cramer P. (2019)
16 more

14 mentions without citation

Processive Antitermination.
Goodson et al. (2018)
13 more