› 6fne

X-ray diffraction
2.5Å resolution

Structure of human Brag2 (Sec7-PH domains) with the inhibitor Bragsin bound to the PH domain

Released:
DOI: 10.2210/pdb6fne/pdb
PDB entry 6fne coloured by chain, front view.
PDB entry 6fne coloured by chain, side view.
PDB entry 6fne coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
PH-domain-binding inhibitors of nucleotide exchange factor BRAG2 disrupt Arf GTPase signaling.
Nawrotek A, Benabdi S, Niyomchon S, Kryszke MH, Ginestier C, Cañeque T, Tepshi L, Mariani A, St Onge RP, Giaever G, Nislow C, Charafe-Jauffret E, Rodriguez R, Zeghouf M, Cherfils J
Nat Chem Biol 15 358-366 (2019)
PMID: 30742123

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179522 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
IQ motif and SEC7 domain-containing protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 405 amino acids
Theoretical weight: 47.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6DN90 (Residues: 512-885; Coverage: 39%)
Gene names: ARFGEP100, BRAG2, IQSEC1, KIAA0763
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand DY5 1 x DY5
Ligand 2PE 1 x 2PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P212121
Unit cell:
a: 65.912Å b: 66.161Å c: 218.823Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.218 0.28
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Inositol Polyphosphate-Based Compounds as Inhibitors of Phosphoinositide 3-Kinase-Dependent Signaling.
Maffucci et al. (2020)
7 other articles

2 mentions without citation

Marked behavioral activation from inhibitory stimulation of locus coeruleus alpha1-adrenoceptors by a full agonist.
Stone et al. (2009)
1 more