› 6fpl

X-ray diffraction
1.6Å resolution

TETR(D) E147A MUTANT IN COMPLEX WITH TETRACYCLINE AND MAGNESIUM

Released:
DOI: 10.2210/pdb6fpl/pdb
Model geometry
Fit model/data
PDB entry 6fpl coloured by chain, front view.
PDB entry 6fpl coloured by chain, side view.
PDB entry 6fpl coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Thermodynamics, cooperativity and stability of the tetracycline repressor (TetR) upon tetracycline binding.
Palm GJ, Buchholz I, Werten S, Girbardt B, Berndt L, Delcea M, Hinrichs W
Biochim Biophys Acta Proteins Proteom 1868 140404 (2020)
PMID: 32114262

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142298 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tetracycline repressor protein class D Chain: A
Molecule details ›
Chain: A
Length: 207 amino acids
Theoretical weight: 23.23 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P0ACT4 (Residues: 2-208; Coverage: 95%)
Gene name: tetR
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand TAC 1 x TAC
Ligand MG 1 x MG
Ligand CL 6 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: I4122
Unit cell:
a: 68.02Å b: 68.02Å c: 179.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.221
Expression system: Escherichia coli K-12

Quick links

Citations

5 citation in other articles

Functional plasticity and evolutionary adaptation of allosteric regulation.
Leander et al. (2020)
4 more