› 6fqq

X-ray diffraction
3.25Å resolution

Crystal structure of TALE homeobox domain transcription factor TGIF1 double alanine mutant bound to its consensus DNA

Released:
DOI: 10.2210/pdb6fqq/pdb
PDB entry 6fqq coloured by chain, front view.
PDB entry 6fqq coloured by chain, side view.
PDB entry 6fqq coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
TGIF1 homeodomain interacts with Smad MH1 domain and represses TGF-β signaling.
Guca E, Suñol D, Ruiz L, Konkol A, Cordero J, Torner C, Aragon E, Martin-Malpartida P, Riera A, Macias MJ
Nucleic Acids Res 46 9220-9235 (2018)
PMID: 30060237

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero pentamer (preferred)
hetero trimer
Assembly name:
PDBe Complex ID:
PDB-CPX-119921 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Homeobox protein TGIF1 Chains: A, B, D, E
Molecule details ›
Chains: A, B, D, E
Length: 71 amino acids
Theoretical weight: 8.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15583 (Residues: 161-229; Coverage: 17%)
Gene names: TGIF, TGIF1
Sequence domains: Homeobox KN domain
Structure domains: Homeodomain-like
DNA (5'-D(P*AP*TP*TP*GP*AP*CP*AP*GP*CP*TP*GP*TP*CP*AP*AP*T)-3') Chains: G, H, L, M
Molecule details ›
Chains: G, H, L, M
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 60.058Å b: 93.016Å c: 100.711Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.215 0.273
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Quo Vadis Biomolecular NMR Spectroscopy?
Selenko P. (2019)
1 more

1 mention without citation

TGIF1 homeodomain interacts with Smad MH1 domain and represses TGF-β signaling.
Guca et al. (2018)