› 6fre

X-ray diffraction
1.22Å resolution

Crystal structure of G-1F/H73A mutant of Ssp DnaB Mini-Intein variant M86

Released:
DOI: 10.2210/pdb6fre/pdb
PDB entry 6fre coloured by chain, front view.
PDB entry 6fre coloured by chain, side view.
PDB entry 6fre coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803 substr. Kazusa
Primary publication:
A functional interplay between intein and extein sequences in protein splicing compensates for the essential block B histidine.
Friedel K, Popp MA, Matern JCJ, Gazdag EM, Thiel IV, Volkmann G, Blankenfeldt W, Mootz HD
Chem Sci 10 239-251 (2019)
PMID: 30713635

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ssp dnaB intein Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 18.91 KDa
Source organism: Synechocystis sp. PCC 6803 substr. Kazusa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q55418 (Residues: 376-486, 767-814; Coverage: 18%)
Gene names: dnaB, slr0833
Sequence domains:
Structure domains: Hedgehog/Intein (Hint) domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P4322
Unit cell:
a: 52.583Å b: 52.583Å c: 118.338Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.175 0.188
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Inteins in Science: Evolution to Application.
Nanda et al. (2020)
2 more

1 mention without citation

A functional interplay between intein and extein sequences in protein splicing compensates for the essential block B histidine.
Friedel et al. (2019)