› 6frg

X-ray diffraction
1.54Å resolution

Crystal structure of G-1F mutant of Ssp DnaB Mini-Intein variant M86

Released:
DOI: 10.2210/pdb6frg/pdb
PDB entry 6frg coloured by chain, front view.
PDB entry 6frg coloured by chain, side view.
PDB entry 6frg coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803 substr. Kazusa
Primary publication:
A functional interplay between intein and extein sequences in protein splicing compensates for the essential block B histidine.
Friedel K, Popp MA, Matern JCJ, Gazdag EM, Thiel IV, Volkmann G, Blankenfeldt W, Mootz HD
Chem Sci 10 239-251 (2019)
PMID: 30713635

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176280 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ssp dnaB intein Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 18.98 KDa
Source organism: Synechocystis sp. PCC 6803 substr. Kazusa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q55418 (Residues: 376-486, 767-814; Coverage: 18%)
Gene names: dnaB, slr0833
Sequence domains:
Structure domains: Hedgehog/Intein (Hint) domain

Ligands and Environments

3 bound ligands:
Ligand 1PE 2 x 1PE
Ligand PGE 1 x PGE
Ligand PEG 4 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P4222
Unit cell:
a: 71.472Å b: 71.472Å c: 87.871Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 0.143 0.17
Expression system: Escherichia coli

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Citations

3 review citations

Inteins in Science: Evolution to Application.
Nanda et al. (2020)
2 more

1 mention without citation

A functional interplay between intein and extein sequences in protein splicing compensates for the essential block B histidine.
Friedel et al. (2019)