› 6frk

Electron Microscopy
3.7Å resolution

Structure of a prehandover mammalian ribosomal SRP and SRP receptor targeting complex

Released:
DOI: 10.2210/pdb6frk/pdb
PDB entry 6frk coloured by chain, front view.
PDB entry 6frk coloured by chain, side view.
PDB entry 6frk coloured by chain, top view.
Source organisms:
Primary publication:
Structure of a prehandover mammalian ribosomal SRP·SRP receptor targeting complex.
Kobayashi K, Jomaa A, Lee JH, Chandrasekar S, Boehringer D, Shan SO, Ban N
Science 360 323-327 (2018)
PMID: 29567807
Related structures: EMD-4300

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 58-mer (preferred)
PDBe Complex ID:
PDB-CPX-300919 (preferred)
Entry contents:
53 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (58 distinct):
Ribosomal protein eL28 Chain: 3
Molecule details ›
Chain: 3
Length: 125 amino acids
Theoretical weight: 14.3 KDa
Source organism: Oryctolagus cuniculus
Ribosomal protein L12 Chain: 4
Molecule details ›
Chain: 4
Length: 163 amino acids
Theoretical weight: 17.69 KDa
Source organism: Oryctolagus cuniculus
60S acidic ribosomal protein P0 Chain: 6
Ribosomal protein L8 Chain: A
Ribosomal protein uL3 Chain: B
Ribosomal protein uL4 Chain: C
Ribosomal protein uL18 Chain: D
Ribosomal protein eL6 Chain: E
Ribosomal protein uL30 Chain: F
Ribosomal protein eL8 Chain: G
Ribosomal protein uL6 Chain: H
Ribosomal protein uL16 Chain: I
Ribosomal protein uL5 Chain: J
Ribosomal protein eL13 Chain: L
Ribosomal protein eL14 Chain: M
Ribosomal protein L15 Chain: N
Ribosomal protein uL13 Chain: O
Ribosomal protein uL22 Chain: P
Ribosomal protein eL18 Chain: Q
Ribosomal protein eL19 Chain: R
Ribosomal protein eL20 Chain: S
Ribosomal protein eL21 Chain: T
Ribosomal protein eL22 Chain: U
Ribosomal protein L23 Chain: V
Ribosomal protein eL24 Chain: W
Ribosomal protein uL23 Chain: X
Ribosomal protein uL24 Chain: Y
60S ribosomal protein L27 Chain: Z
Ribosomal protein uL15 Chain: a
60S ribosomal protein L29 Chain: b
Ribosomal protein eL30 Chain: c
Ribosomal protein eL31 Chain: d
Ribosomal protein eL32 Chain: e
Ribosomal protein eL33 Chain: f
Ribosomal protein eL34 Chain: g
Ribosomal protein uL29 Chain: h
Ribosomal protein eL36 Chain: i
Ribosomal protein L37 Chain: j
Ribosomal protein eL38 Chain: k
Ribosomal protein eL39 Chain: l
Ribosomal protein eL40 Chain: m
Ribosomal protein eL41 Chain: n
Ribosomal protein eL42 Chain: o
Ribosomal protein eL43 Chain: p
Signal recognition particle 19 kDa protein Chain: q
Signal recognition particle subunit SRP72 Chain: r
Signal sequence Chain: t
Signal recognition particle subunit SRP68 Chain: u
Signal recognition particle receptor subunit beta Chain: v
Signal recognition particle 9 kDa protein Chain: w
Signal recognition particle subunit SRP54 Chain: x
SRP54-type proteins GTP-binding domain-containing protein Chain: y
Signal recognition particle 14 kDa protein Chain: z
Canis lupus familiaris RNA, 7SL, cytoplasmic 1 (RN7SL1), SRP RNA Chain: 1
tRNA Chain: 2
28S ribosomal RNA Chain: 5
5S ribosomal RNA Chain: 7
5.8S ribosomal RNA Chain: 8

Ligands and Environments

4 bound ligands:
Ligand MG 133 x MG
Ligand ZN 3 x ZN
Ligand GTP 1 x GTP
Ligand GNP 2 x GNP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.7Å
Relevant EMDB volumes: EMD-4300
Expression systems:
  • Oryctolagus cuniculus
  • Escherichia coli

Quick links

Citations

7 review citations

The mechanisms of integral membrane protein biogenesis.
Hegde et al. (2022)
6 more

8 mentions without citation

Noncanonical Functions and Cellular Dynamics of the Mammalian Signal Recognition Particle Components.
Faoro et al. (2021)
7 more