› 6frn

X-ray diffraction
1.74Å resolution

Structure of F420H2 oxidase (FprA) co-crystallized with 10mM Tb-Xo4 and calcium chloride

Released:
DOI: 10.2210/pdb6frn/pdb
PDB entry 6frn coloured by chain, front view.
PDB entry 6frn coloured by chain, side view.
PDB entry 6frn coloured by chain, top view.
Source organism: Methanothermococcus thermolithotrophicus
Primary publication:
Unveiling the Binding Modes of the Crystallophore, a Terbium-based Nucleating and Phasing Molecular Agent for Protein Crystallography.
Engilberge S, Riobé F, Wagner T, Di Pietro S, Breyton C, Franzetti B, Shima S, Girard E, Dumont E, Maury O
Chemistry 24 9739-9746 (2018)
PMID: 29806881

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105067 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavodoxin-like domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 410 amino acids
Theoretical weight: 46.24 KDa
Source organism: Methanothermococcus thermolithotrophicus
UniProt:
  • Canonical: A0A452CSW8 (Residues: 1-410; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

7 bound ligands:
Ligand FMN 4 x FMN
Ligand CA 15 x CA
Ligand TB 4 x TB
Ligand GOL 6 x GOL
Ligand PE4 6 x PE4
Ligand 7MT 4 x 7MT
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 73.66Å b: 144.97Å c: 74.21Å
α: 90° β: 91.78° γ: 90°
R-values:
R R work R free
0.166 0.164 0.189

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Citations

1 review citation

NMR Spectroscopy of supramolecular chemistry on protein surfaces.
Bayer et al. (2020)
1 other article