› 6fsq

X-ray diffraction
2.79Å resolution

Structure of A3_bGFPD, an artificial bi-domain protein based on two different alphaRep domains : A3 and a GFP binding domain (bGFPD)

Released:
DOI: 10.2210/pdb6fsq/pdb
Model geometry
Fit model/data
PDB entry 6fsq coloured by chain, front view.
PDB entry 6fsq coloured by chain, side view.
PDB entry 6fsq coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
Ligand-induced conformational switch in an artificial bidomain protein scaffold.
Léger C, Di Meo T, Aumont-Nicaise M, Velours C, Durand D, Li de la Sierra-Gallay I, van Tilbeurgh H, Hildebrandt N, Desmadril M, Urvoas A, Valerio-Lepiniec M, Minard P
OpenAccess logo Sci Rep 9 1178 (2019)
PMID: 30718544

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166735 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
alphaRep A3_bGFPD Chain: A
Molecule details ›
Chain: A
Length: 409 amino acids
Theoretical weight: 44.46 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand MLI 1 x MLI
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2221
Unit cell:
a: 66.94Å b: 89.03Å c: 143.61Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.27
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Aptamer and nanomaterial based FRET biosensors: a review on recent advances (2014-2019).
Pehlivan et al. (2019)
5 other articles