› 6ft7

X-ray diffraction
2.02Å resolution

Crystal structure of CLK3 in complex with compound 8a

Released:
DOI: 10.2210/pdb6ft7/pdb
PDB entry 6ft7 coloured by chain, front view.
PDB entry 6ft7 coloured by chain, side view.
PDB entry 6ft7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular structures of cdc2-like kinases in complex with a new inhibitor chemotype.
Walter A, Chaikuad A, Helmer R, Loaëc N, Preu L, Ott I, Knapp S, Meijer L, Kunick C
PLoS One 13 e0196761 (2018)
PMID: 29723265

Function and Biology Details

Reaction catalysed: 
ATP + L-seryl/L-threonyl/L-tyrosyl-[protein] = ADP + O-phospho-L-seryl/O-phospho-L-threonyl/O-phospho-L-tyrosyl-[protein]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156013 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dual specificity protein kinase CLK3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 360 amino acids
Theoretical weight: 42.32 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49761 (Residues: 275-632; Coverage: 56%)
Gene name: CLK3
Sequence domains: Protein kinase domain
Structure domains: Phosphorylase Kinase; domain 1

Ligands and Environments

3 bound ligands:
Ligand IOD 14 x IOD
Ligand EDO 12 x EDO
Ligand E6Q 2 x E6Q
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 61.76Å b: 116.83Å c: 69.91Å
α: 90° β: 92.75° γ: 90°
R-values:
R R work R free
0.194 0.192 0.235
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Targeting mRNA processing as an anticancer strategy.
Desterro et al. (2020)
3 more

2 mentions without citation

Dual-Specificity, Tyrosine Phosphorylation-Regulated Kinases (DYRKs) and cdc2-Like Kinases (CLKs) in Human Disease, an Overview.
Lindberg et al. (2021)
1 more