› 6ftf

X-ray diffraction
1.09Å resolution

Regulatory subunit of a cAMP-independent protein kinase A from Trypanosoma cruzi at 1.09 A resolution

Released:
DOI: 10.2210/pdb6ftf/pdb
PDB entry 6ftf coloured by chain, front view.
PDB entry 6ftf coloured by chain, side view.
PDB entry 6ftf coloured by chain, top view.
Source organism: Trypanosoma cruzi strain CL Brener
Primary publication:
Nucleoside analogue activators of cyclic AMP-independent protein kinase A of Trypanosoma.
Bachmaier S, Volpato Santos Y, Kramer S, Githure GB, Klöckner T, Pepperl J, Baums C, Schenk R, Schwede F, Genieser HG, Dupuy JW, Forné I, Imhof A, Basquin J, Lorentzen E, Boshart M
Nat Commun 10 1421 (2019)
PMID: 30926779

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175486 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclic nucleotide-binding domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 304 amino acids
Theoretical weight: 34.48 KDa
Source organism: Trypanosoma cruzi strain CL Brener
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q4DSV5 (Residues: 200-503; Coverage: 60%)
Gene name: Tc00.1047053506227.150
Sequence domains: Cyclic nucleotide-binding domain

Ligands and Environments

2 bound ligands:
Ligand EDO 3 x EDO
Ligand 7CI 2 x 7CI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 40.846Å b: 89.066Å c: 45.634Å
α: 90° β: 98.182° γ: 90°
R-values:
R R work R free
0.151 0.15 0.16
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

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Citations

6 review citations

Who Needs a Contractile Actomyosin Ring? The Plethora of Alternative Ways to Divide a Protozoan Parasite.
Hammarton TC. (2019)
5 more