› 6fuu

X-ray diffraction
1.75Å resolution

Transcriptional regulator LmrR with bound heme

Released:
DOI: 10.2210/pdb6fuu/pdb
PDB entry 6fuu coloured by chain, front view.
PDB entry 6fuu coloured by chain, side view.
PDB entry 6fuu coloured by chain, top view.
Source organism: Lactococcus cremoris
Primary publication:
An Artificial Heme Enzyme for Cyclopropanation Reactions.
Villarino L, Splan KE, Reddem E, Alonso-Cotchico L, Gutiérrez de Souza C, Lledós A, Maréchal JD, Thunnissen AWH, Roelfes G
Angew Chem Int Ed Engl 57 7785-7789 (2018)
PMID: 29719099

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-241465 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulator PadR family Chain: A
Molecule details ›
Chain: A
Length: 126 amino acids
Theoretical weight: 14.76 KDa
Source organism: Lactococcus cremoris
Expression system: Escherichia coli
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

1 bound ligand:
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P43212
Unit cell:
a: 35.145Å b: 35.145Å c: 180.668Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.237
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Artificial Metalloenzymes: Challenges and Opportunities.
Davis et al. (2019)
4 more

3 mentions without citation

LmrR: A Privileged Scaffold for Artificial Metalloenzymes.
Roelfes G. (2019)
2 more