› 6fv0

X-ray diffraction
2.29Å resolution

Crystal structure of the TPR domain of KLC1 in complex with the C-terminal peptide of torsinA

Released:
DOI: 10.2210/pdb6fv0/pdb
PDB entry 6fv0 coloured by chain, front view.
PDB entry 6fv0 coloured by chain, side view.
PDB entry 6fv0 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for isoform-specific kinesin-1 recognition of Y-acidic cargo adaptors.
Pernigo S, Chegkazi MS, Yip YY, Treacy C, Glorani G, Hansen K, Politis A, Bui S, Dodding MP, Steiner RA
Elife 7 (2018)
PMID: 30320553

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208159 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Kinesin light chain 1; Torsin-1A Chain: A
Molecule details ›
Chain: A
Length: 338 amino acids
Theoretical weight: 37.45 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O88447 (Residues: 204-495; Coverage: 54%)
  • Canonical: Q9ER39 (Residues: 322-333; Coverage: 4%)
Gene names: Dyt1, Klc1, Kns2, Tor1a
Sequence domains:
nanobody Chain: F
Molecule details ›
Chain: F
Length: 121 amino acids
Theoretical weight: 13.4 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2
Unit cell:
a: 106.04Å b: 89.68Å c: 50.99Å
α: 90° β: 98° γ: 90°
R-values:
R R work R free
0.21 0.208 0.245
Expression systems:
  • Escherichia coli BL21
  • Escherichia coli

Quick links

Citations

11 citation in other articles

Binding and transport of SFPQ-RNA granules by KIF5A/KLC1 motors promotes axon survival.
Fukuda et al. (2021)
10 more

2 mentions without citation

Structural basis for isoform-specific kinesin-1 recognition of Y-acidic cargo adaptors.
Pernigo et al. (2018)
1 more