› 6fw2

X-ray diffraction
1.78Å resolution

Crystal Structure of human mARC1

Released:
DOI: 10.2210/pdb6fw2/pdb
PDB entry 6fw2 coloured by chain, front view.
PDB entry 6fw2 coloured by chain, side view.
PDB entry 6fw2 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of human mARC1 reveals its exceptional position among eukaryotic molybdenum enzymes.
Kubitza C, Bittner F, Ginsel C, Havemeyer A, Clement B, Scheidig AJ
Proc Natl Acad Sci U S A 115 11958-11963 (2018)
PMID: 30397129

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133012 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin; Mitochondrial amidoxime-reducing component 1 Chain: A
Molecule details ›
Chain: A
Length: 459 amino acids
Theoretical weight: 51.85 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: Q5VT66 (Residues: 53-128, 131-336; Coverage: 84%)
  • Canonical: P00720 (Residues: 1-159; Coverage: 97%)
Gene names: E, MARC1, MOSC1, MTARC1
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand MTE 1 x MTE
Ligand EFK 1 x EFK
Ligand B3P 1 x B3P
Ligand MOO 4 x MOO
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: P212121
Unit cell:
a: 61.133Å b: 74.818Å c: 110.726Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.169 0.208
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Molybdenum Enzymes and How They Support Virulence in Pathogenic Bacteria.
Zhong et al. (2020)
6 more

4 mentions without citation

The mitochondrial amidoxime reducing component-from prodrug-activation mechanism to drug-metabolizing enzyme and onward to drug target.
Struwe et al. (2023)
3 more