› 6fwx

X-ray diffraction
3Å resolution

Chimeric titin Z1Z2-Z1Z2 tandem (Z1212) functionalized with a GRGDS exogenous peptide from fibronectin

Released:
DOI: 10.2210/pdb6fwx/pdb
PDB entry 6fwx coloured by chain, front view.
PDB entry 6fwx coloured by chain, side view.
PDB entry 6fwx coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Self-Assembling Proteins as High-Performance Substrates for Embryonic Stem Cell Self-Renewal.
Hill CJ, Fleming JR, Mousavinejad M, Nicholson R, Tzokov SB, Bullough PA, Bogomolovas J, Morgan MR, Mayans O, Murray P
Adv Mater 31 e1807521 (2019)
PMID: 30866118

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186809 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Titin Chains: A, B
Molecule details ›
Chains: A, B
Length: 394 amino acids
Theoretical weight: 41.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8WZ42 (Residues: 1-47, 52-196; Coverage: 1%)
Gene name: TTN
Sequence domains: Immunoglobulin I-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: R3
Unit cell:
a: 134.4Å b: 134.4Å c: 134Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.2 0.226
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

MgFe-LDH Nanoparticles: A Promising Leukemia Inhibitory Factor Replacement for Self-Renewal and Pluripotency Maintenance in Cultured Mouse Embryonic Stem Cells.
He et al. (2021)

2 mentions without citation

The ZT Biopolymer: A Self-Assembling Protein Scaffold for Stem Cell Applications.
Nesterenko et al. (2019)
1 more