› 6fwy

X-ray diffraction
2.14Å resolution

Thioester domain of the Enterococcus faecium TIE86 protein

Released:
DOI: 10.2210/pdb6fwy/pdb
PDB entry 6fwy coloured by chain, front view.
PDB entry 6fwy coloured by chain, side view.
PDB entry 6fwy coloured by chain, top view.
Source organism: Enterococcus faecium
Primary publication:
A new structural class of bacterial thioester domains reveals a slipknot topology.
Miller OK, Banfield MJ, Schwarz-Linek U
Protein Sci 27 1651-1660 (2018)
PMID: 30052296

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103210 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
B-type Cna protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 280 amino acids
Theoretical weight: 30.37 KDa
Source organism: Enterococcus faecium
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1A7T0E1 (Residues: 39-314; Coverage: 35%)
Gene name: B4W81_16995
Sequence domains: Thioester domain

Ligands and Environments

2 bound ligands:
Ligand PG4 1 x PG4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 197.7Å b: 33.732Å c: 206.052Å
α: 90° β: 105.88° γ: 90°
R-values:
R R work R free
0.234 0.233 0.258
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The long and the short of Periscope Proteins.
Whelan F. (2022)
1 more

2 mentions without citation

A new structural class of bacterial thioester domains reveals a slipknot topology.
Miller et al. (2018)
1 more