› 6fx6

X-ray diffraction
2.25Å resolution

Thioester domain of the Staphylococcus aureus TIE protein

Released:
DOI: 10.2210/pdb6fx6/pdb
PDB entry 6fx6 coloured by chain, front view.
PDB entry 6fx6 coloured by chain, side view.
PDB entry 6fx6 coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
A new structural class of bacterial thioester domains reveals a slipknot topology.
Miller OK, Banfield MJ, Schwarz-Linek U
Protein Sci 27 1651-1660 (2018)
PMID: 30052296

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104856 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thioester domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 28.48 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A3F2YM24 (Residues: 1-253; Coverage: 100%)
Sequence domains: Thioester domain

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand ACT 2 x ACT
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P212121
Unit cell:
a: 44.029Å b: 73.612Å c: 74.651Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The long and the short of Periscope Proteins.
Whelan F. (2022)
1 more

2 mentions without citation

A new structural class of bacterial thioester domains reveals a slipknot topology.
Miller et al. (2018)
1 more