› 6fxe

X-ray diffraction
2.4Å resolution

BBE31 from Lyme disease agent Borrelia (Borreliella) burgdorferi playing a vital role in successful colonization of the mammalian host

Released:
DOI: 10.2210/pdb6fxe/pdb
PDB entry 6fxe coloured by chain, front view.
PDB entry 6fxe coloured by chain, side view.
PDB entry 6fxe coloured by chain, top view.
Source organism: Borreliella burgdorferi B31
Primary publication:
BBE31 from the Lyme disease agent Borrelia burgdorferi, known to play an important role in successful colonization of the mammalian host, shows the ability to bind glutathione.
Brangulis K, Akopjana I, Petrovskis I, Kazaks A, Zelencova D, Jekabsons A, Jaudzems K, Tars K
Biochim Biophys Acta Gen Subj 1864 129499 (2020)
PMID: 31785327

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129336 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Surface protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 210 amino acids
Theoretical weight: 24.26 KDa
Source organism: Borreliella burgdorferi B31
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O50725 (Residues: 38-243; Coverage: 85%)
Gene name: BB_E31
Sequence domains: Borrelia Bbcrasp-1 domain containing protein

Ligands and Environments

1 bound ligand:
Ligand GSH 2 x GSH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 74.39Å b: 75.27Å c: 87.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.259
Expression system: Escherichia coli BL21

Quick links

Citations

4 citation in other articles

Interactions Between Ticks and Lyme Disease Spirochetes.
Pal et al. (2021)
3 more

1 mention without citation

The surface lipoproteins of gram-negative bacteria: Protectors and foragers in harsh environments.
Cole et al. (2021)