› 6fy8

X-ray diffraction
3Å resolution

The crystal structure of EncM bromide soak

Released:
DOI: 10.2210/pdb6fy8/pdb
PDB entry 6fy8 coloured by chain, front view.
PDB entry 6fy8 coloured by chain, side view.
PDB entry 6fy8 coloured by chain, top view.
Source organism: Streptomyces maritimus
Primary publication:
Enzymatic control of dioxygen binding and functionalization of the flavin cofactor.
Saleem-Batcha R, Stull F, Sanders JN, Moore BS, Palfey BA, Houk KN, Teufel R
Proc Natl Acad Sci U S A 115 4909-4914 (2018)
PMID: 29686059

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191887 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD-binding PCMH-type domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 464 amino acids
Theoretical weight: 50.07 KDa
Source organism: Streptomyces maritimus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KHK2 (Residues: 1-464; Coverage: 100%)
Gene name: encM
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FAD 1 x FAD
Ligand BR 2 x BR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: I222
Unit cell:
a: 82.241Å b: 87.172Å c: 130.909Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.241 0.287
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Enzymatic Cascade Reactions in Biosynthesis.
Walsh et al. (2019)
3 more