› 6fyf

X-ray diffraction
2.2Å resolution

The crystal structure of EncM V135M mutant

Released:
DOI: 10.2210/pdb6fyf/pdb
Model geometry
Fit model/data
PDB entry 6fyf coloured by chain, front view.
PDB entry 6fyf coloured by chain, side view.
PDB entry 6fyf coloured by chain, top view.
Source organism: Streptomyces maritimus
Primary publication:
Enzymatic control of dioxygen binding and functionalization of the flavin cofactor.
Saleem-Batcha R, Stull F, Sanders JN, Moore BS, Palfey BA, Houk KN, Teufel R
Proc Natl Acad Sci U S A 115 4909-4914 (2018)
PMID: 29686059

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191887 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD-binding PCMH-type domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 464 amino acids
Theoretical weight: 50.11 KDa
Source organism: Streptomyces maritimus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KHK2 (Residues: 1-464; Coverage: 100%)
Gene name: encM
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand FAD 4 x FAD
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C222
Unit cell:
a: 162.268Å b: 175.164Å c: 130.405Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.225 0.274
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Enzymatic Cascade Reactions in Biosynthesis.
Walsh et al. (2019)
3 more