› 6fzd

X-ray diffraction
1.8Å resolution

Crystal Structure of lipase from Geobacillus stearothermophilus T6 variant L184F/A187F/L360F

Released:
DOI: 10.2210/pdb6fzd/pdb
PDB entry 6fzd coloured by chain, front view.
PDB entry 6fzd coloured by chain, side view.
PDB entry 6fzd coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Filling the Void: Introducing Aromatic Interactions into Solvent Tunnels To Enhance Lipase Stability in Methanol.
Gihaz S, Kanteev M, Pazy Y, Fishman A
Appl Environ Microbiol 84 (2018)
PMID: 30217852

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188034 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
triacylglycerol lipase Chain: A
Molecule details ›
Chain: A
Length: 392 amino acids
Theoretical weight: 44.08 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93A71 (Residues: 33-418; Coverage: 92%)

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P212121
Unit cell:
a: 49.86Å b: 70.881Å c: 113.167Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.212 0.229
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Thermostability engineering of industrial enzymes through structure modification.
Nezhad et al. (2022)
4 other articles