› 6fzt

X-ray diffraction
2.46Å resolution

Crystal structure of Smad8_9-MH1 bound to the GGCGC site.

Released:
DOI: 10.2210/pdb6fzt/pdb
PDB entry 6fzt coloured by chain, front view.
PDB entry 6fzt coloured by chain, side view.
PDB entry 6fzt coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Unveiling the dimer/monomer propensities of Smad MH1-DNA complexes
Ruiz L, Kaczmarska Z, Gomes T, Aragon E, Torner C, Freier R, Baginski B, Martin-Malpartida P, Marquez JA, Cordeiro TN, Pluta R, Macias MJ
Computational and Structural Biotechnology Journal 19 (2021)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-119932 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Mothers against decapentaplegic homolog 9 Chains: A, B
Molecule details ›
Chains: A, B
Length: 140 amino acids
Theoretical weight: 16.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O15198 (Residues: 1-140; Coverage: 30%)
Gene names: MADH6, MADH9, SMAD8, SMAD9
Sequence domains: MH1 domain
DNA (5'-D(P*TP*GP*CP*AP*GP*GP*CP*GP*CP*GP*CP*CP*TP*GP*CP*A)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE MASSIF-3
Spacegroup: P212121
Unit cell:
a: 75.433Å b: 79.509Å c: 88.372Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.237
Expression systems:
  • Escherichia coli
  • Not provided

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