› 6g0l

Electron Microscopy
10Å resolution

Structure of two molecules of the chromatin remodelling enzyme Chd1 bound to a nucleosome

Released:
DOI: 10.2210/pdb6g0l/pdb
PDB entry 6g0l coloured by chain, front view.
PDB entry 6g0l coloured by chain, side view.
PDB entry 6g0l coloured by chain, top view.
Source organisms:
Primary publication:
Structure of the chromatin remodelling enzyme Chd1 bound to a ubiquitinylated nucleosome.
Sundaramoorthy R, Hughes AL, El-Mkami H, Norman DG, Ferreira H, Owen-Hughes T
Elife 7 (2018)
PMID: 30079888
Related structures: EMD-4336

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-119934 (preferred)
Entry contents:
6 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (8 distinct):
Histone H3 Chains: A, E
Molecule details ›
Chains: A, E
Length: 136 amino acids
Theoretical weight: 15.41 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92133 (Residues: 1-136; Coverage: 100%)
Gene names: H3l, Hist1h3f, h3, h3.2a, h3c8, h3c8.S, h3r, hist1h3g, hist1h3g.L, hist2h3, hist2h3c
Sequence domains: Core histone H2A/H2B/H3/H4
Histone H4 Chains: B, F
Molecule details ›
Chains: B, F
Length: 103 amino acids
Theoretical weight: 11.39 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P62799 (Residues: 1-103; Coverage: 100%)
Sequence domains: Centromere kinetochore component CENP-T histone fold
Histone H2A type 1 Chain: C
Molecule details ›
Chain: C
Length: 130 amino acids
Theoretical weight: 14.19 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P06897 (Residues: 1-130; Coverage: 100%)
Sequence domains:
Histone H4 Chains: D, H
Molecule details ›
Chains: D, H
Length: 126 amino acids
Theoretical weight: 13.94 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
Histone H2A type 1 Chain: G
Molecule details ›
Chain: G
Length: 130 amino acids
Theoretical weight: 14.09 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P06897 (Residues: 1-130; Coverage: 100%)
Sequence domains:
Chromo domain-containing protein 1 Chains: M, W
Molecule details ›
Chains: M, W
Length: 1468 amino acids
Theoretical weight: 168.5 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P32657 (Residues: 1-1468; Coverage: 100%)
Gene names: CHD1, SYGP-ORF4, YER164W
Sequence domains:
DNA (176-MER) Chain: I
Molecule details ›
Chain: I
Length: 176 nucleotides
Theoretical weight: 54.09 KDa
DNA (177-MER) Chain: J
Molecule details ›
Chain: J
Length: 177 nucleotides
Theoretical weight: 54.89 KDa

Ligands and Environments

2 bound ligands:
Ligand ADP 2 x ADP
Ligand BEF 2 x BEF
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 10Å
Relevant EMDB volumes: EMD-4336
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Principles of nucleosome recognition by chromatin factors and enzymes.
McGinty et al. (2021)
12 more