› 6g0z

X-ray diffraction
2.15Å resolution

Crystal structure of GDP bound RbgA from S. aureus

Released:
DOI: 10.2210/pdb6g0z/pdb
PDB entry 6g0z coloured by chain, front view.
PDB entry 6g0z coloured by chain, side view.
PDB entry 6g0z coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus USA300
Primary publication:
Structural basis for (p)ppGpp-mediated inhibition of the GTPase RbgA.
Pausch P, Steinchen W, Wieland M, Klaus T, Freibert SA, Altegoer F, Wilson DN, Bange G
J Biol Chem 293 19699-19709 (2018)
PMID: 30366986

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101659 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosome biogenesis GTPase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 301 amino acids
Theoretical weight: 34.32 KDa
Source organism: Staphylococcus aureus subsp. aureus USA300
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2XK72 (Residues: 2-294; Coverage: 100%)
Gene name: SAUSA300_1136
Sequence domains: 50S ribosome-binding GTPase

Ligands and Environments

1 bound ligand:
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P212121
Unit cell:
a: 71.932Å b: 77.812Å c: 124.667Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

The Impact of the Stringent Response on TRAFAC GTPases and Prokaryotic Ribosome Assembly.
Bennison et al. (2019)
3 more

1 mention without citation

Structural basis for (p)ppGpp-mediated inhibition of the GTPase RbgA.
Pausch et al. (2018)