› 6g1b

X-ray diffraction
2.28Å resolution

Corynebacterium glutamicum OxyR, oxidized form

Released:
DOI: 10.2210/pdb6g1b/pdb
PDB entry 6g1b coloured by chain, front view.
PDB entry 6g1b coloured by chain, side view.
PDB entry 6g1b coloured by chain, top view.
Source organism: Corynebacterium glutamicum
Primary publication:
Structural snapshots of OxyR reveal the peroxidatic mechanism of H2O2 sensing.
Pedre B, Young D, Charlier D, Mourenza Á, Rosado LA, Marcos-Pascual L, Wahni K, Martens E, G de la Rubia A, Belousov VV, Mateos LM, Messens J
Proc Natl Acad Sci U S A 115 E11623-E11632 (2018)
PMID: 30463959

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185623 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Probable hydrogen peroxide-inducible genes activator Chain: J
Molecule details ›
Chain: J
Length: 327 amino acids
Theoretical weight: 35.09 KDa
Source organism: Corynebacterium glutamicum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NP91 (Residues: 1-324; Coverage: 99%)
Gene name: Cgl1925
Sequence domains:
Probable hydrogen peroxide-inducible genes activator Chain: B
Molecule details ›
Chain: B
Length: 326 amino acids
Theoretical weight: 35 KDa
Source organism: Corynebacterium glutamicum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NP91 (Residues: 1-323; Coverage: 99%)
Gene name: Cgl1925
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand NI 1 x NI
Ligand FMT 3 x FMT
Ligand 1PS 1 x 1PS
Ligand NA 1 x NA
Ligand PEO 3 x PEO
1 modified residue:
Ligand SME 1 x SME

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: C2
Unit cell:
a: 161.307Å b: 46.186Å c: 116.692Å
α: 90° β: 121.92° γ: 90°
R-values:
R R work R free
0.198 0.196 0.23
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Looking Back at the Early Stages of Redox Biology.
Flohé L. (2020)
6 more

3 mentions without citation

How Bacterial Redox Sensors Transmit Redox Signals via Structural Changes.
Lee et al. (2021)
2 more