› 6g29

X-ray diffraction
1.7Å resolution

Function and Biology Details

Reactions catalysed: 
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(4) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(4)
(1a) S-adenosyl-L-methionine + a [histone H3]-L-lysine(27) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(27)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189853 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone-lysine N-methyltransferase NSD3 Chain: A
Molecule details ›
Chain: A
Length: 136 amino acids
Theoretical weight: 16.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9BZ95 (Residues: 263-398; Coverage: 10%)
Gene names: DC28, NSD3, WHSC1L1
Sequence domains: PWWP domain

Ligands and Environments

1 bound ligand:
Ligand EHK 1 x EHK
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 44.003Å b: 47.431Å c: 64.287Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.195 0.21
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Using Chemical Epigenetics to Target Cancer.
Wimalasena et al. (2020)
13 more